Authors: S. Stead, S. Richmond, M. Sharman, J. Stark, E. Geijp
Manufacturer: Copyright (c) 2006 Elsevier B.V.
A rapid,
high-throughput antimicrobial screening assay has been developed that combines either a physical fluid extraction or a solvent extraction technique with the
commercially available Premi^(R)Test. In order to remove the subjectivity of the visual end-point measurement associated with this microbial inhibition assay, work has been conducted to couple the Premi^(R)Test to scanner technology. The use of the solvent extraction provides an enhanced detection
capability for a wide range of drugs at or below one-half the maximum residue limit (MRL) concentrations in a variety of matrices, as demonstrated by dose response curve data. Secondary class-specific assays, for the identification of