Authors: P. Roychoudhury, L.M. Harvey, B. McNeil
Manufacturer: Copyright (c) 2006 Elsevier B.V.
The utility of attenuated total
reflectance-mid-infrared spectroscopy (ATR-MIR) to monitor the concentrations of the key analytes ammonium, glucose, methyl oleate and biomass in a
Streptomyces clavuligerus
bioprocess for the synthesis of clavulanic acid was investigated. The cultivation medium was complex and the process fluid (matrix) underwent profound changes as culture growth proceeded, representing a considerable challenge from a spectroscopic viewpoint. Quantitative models were developed using the multivariate statistical technique, partial least square (PLS) for the key analytes over the entire time course of the bioprocess, which were validated externally using samples not