Authors: C. Benetti, N. Dainese, G. Biancotto, R. Piro, F. Mutinelli
Manufacturer: Copyright (c) 2006 Elsevier B.V.
A liquid chromatographic-tandem mass spectrometric (HPLC-MS/MS) method is
proposed for the identification and
quantification of tylosin in honey. Sample treatment involves an
extraction in a Tris buffer at pH 10.5, followed by a solid-phase clean up step on an Oasis HLB column. Roxithromycin was used as the internal standard. Chromatographic separation of tylosin and roxithromycin was performed on an XTerra MS C"1"8 column (100mm x 2.1mm i.d., 5@mm) using a gradient of aqueous 0.01M ammonium acetate pH 3.5 and acetonitrile as the mobile phase, at a flow rate of 0.25mlmin^-^1. The method was validated according to the guidelines laid down by the Commission Decision 2002/657/EC. Tylosin…
