Authors: C. Situ, C.T. Elliott
Manufacturer: Copyright (c) 2006 Elsevier B.V.
As a result of increasing concerns over the transfer of resistance between different bacteria and between human and animals, a group of
antimicrobial growth promoters including bacitracin, spiramycin, tylosin, virginiamycin and olaquindox have been banned in the EU since 1999. A rapid and sensitive
enzyme-linked immunosorbent assay (ELISA) with simple extraction procedures was developed for simultaneous screening of the five banned feed additives in
animal feeds. Banned substances were extracted from animal feeds with 70% methanol in water followed by clean-up on OASIS^(R) HLB cartridges. Polyclonal (Pab) antibodies were generated and their specificities assessed by cross-reactivity studies…
