Authors: M. Hu, C.Q. Hu
Manufacturer: Copyright (c) 2006 Elsevier B.V.
We describe a method using
liquid chromatography combined with positive electrospray ionization
tandem mass spectrometry (LC/ESI-MS/MS) to identify the components of 16-membered macrolide antibiotics. Choosing three characteristic compounds (midecamycin A"1, midecamycin acetate, 4''-isovalerylspiramycin III), the mass fragment pathway of this group of antibiotics was determined. The characteristics of these kinds of compounds on chromatographic behavior and UV spectrum were revealed and confirmed by the components in six examples (kitasamycin, midecamycin, meleumycin, josamycin, acetylspiramycin, 4''-acylspiramycin), whose structure were already known.
Utilizing this method, some components