Author: Eugenio H. Zabaleta
The aim of this work was to develop an
Enzyme-Linked ImmunoSorbent Assay (ELISA) to be used for agmatine Therapeutic
Drug Monitoring (TDM) when administrated to patients for neuropathic pain or in neurological research to understand the role of agmatine as a neurotransmitter in the central
nervous system. The ELISA was developed and improved in a series of six trials. In the first, we attempted to prove that an ELISA for agmatine was feasible for the range found in human plasma, as well as optimize the concentrations of reagents to work more accurately in that range. In the second, third, fourth and fifth trials we tried to improve the ELISA, making it shorter, easier, more user friendly and…
