Lysobacter enzymogenes strain C3 is a
biological control agent against
fungal pathogens. Its activity was attributed previously to multiple antifungal lytic enzymes. In this study, a heat stable antibiotic (HSAF) was also found to be responsible for antifungal activity in vitro. HSAF was extracted from C3 culture fluid by ammonium sulfate precipitation and solubilization in methanol and was further isolated by TLC and HPLC. The purified antibiotic was active against a wide range of fungal and oomycete species but not against
Saccharomyces cerevisiae or bacteria. It inhibited spore germination and disrupted hyphal polarity in vitro. These effects also were observed when HSAF was applied to…
