Gelatin-methotrexate (G-MTX) conjugates have potential advantages over free drug therapy including reduced systemic
cytotoxicity and the ability to overcome MTX resistance. The effect of drug load on degradation and release from G-MTX conjugates under in vitro lysosomal and
interstitial tumor conditions was evaluated. G-MTX conjugates were crosslinked together into microspheres (C-G-MTX MCS) to form a drug delivery system for direct injection into the tumor site that would act as a controlled release depot for gelatin-MTX fragments (
low molecular weight conjugates). The effect of drug load on release and degradation of these C-G-MTX MCS under in vitro tumor conditions was evaluated.
