Authors: M. El Shafie, Mario Sousa, Thunus F. Kruger
This ground-breaking atlas of oocyte ultrastructure forms a practical guide to assisted reproduction. Extensively illustrated with
transmission electron microscopic (TEM) images, it contains chapters on oocyte maturation, fertilization, fertilization failure, pitfalls and technical problems of intracytoplasmic sperm injection, and failed
fertilization in vitro. A large chapter of TEM images illustrates fertilization failures after ICSI and IVF and the ultrastructure of germinal vesicle oocytes and metaphase I oocytes. The concluding chapter describes materials and methods for single-cell
transmission electron microscopy of the human oocyte.